It is always that third reviewer (well, actually in my case it was the second reviewer). That one that can just kill ya.
You know the one I mean. The one that said that you did the assay ALL wrong, the assay you’ve been doing for 20 years and can produce at least 15 references from top labs in your field that support the method that you used as perfectly correct. Uh huh. Or the one that uses clearly condescending language- like… THANK GOD they decided to do XYZ (implies… at least one of the authors over there knows what they are doing!). Or how about the … you didn’t cite my work… disguised as ‘the authors should correct an egregious omission of the work referencing bla bla bla by famous scientist X. These references should be cited on in the relevant section’. Ok sometimes that one is for real. Or better yet- you didn’t cite the biology I work on, even though it is only peripheral to the biology that you put in this paper. Or how about the reviewer that seems to have trouble integrating panel A with the controls in panel B, and keeps claiming that your image is an artifact of your technique, even though your experimental sample and your control sample use the same technique and the results have been quantified and are clearly statistically significantly different. Finally… there is the reviewer that complains endlessly about the poor grammar and spelling … in a review that is filled with spelling and grammatical errors. (and just so you know, I may have made any or all of these points at one time or another…. although I hope that I did not).
Name your favorite cranky reviewer stock review points.